Anti-VprBP; clone 8A7.2

Code: MABE969 D2-231

Application

Research CategoryEpigenetics

Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected VprBP in human kidney, human small intest...


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Application

Research CategoryEpigenetics

Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected VprBP in human kidney, human small intestine, and rat testis tissue sections.

Anti-VprBP Antibody, clone 8A7.2 is an antibody against VprBP for use in Western Blotting, Immunohistochemistry (Paraffin).

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein VPRBP (EC 2.7.11.1; UniProt Q9Y4B6; also known as DDB1- and CUL4-associated factor 1, HIV-1 Vpr-binding protein, Serine/threonine-protein kinase VPRBP, Vpr-interacting protein, VprBP) is encoded by the VPRBP (also known as DCAF1, KIAA0800, RIP) gene (Gene ID 9730) in human. VprBP/DCAF1 acts as an adaptor molecule that mediates substrates recruitment for both the RING-type CRL4 (Cul4-Roc1-DDB1) and HECT-type EDD/UBR5 E3 ubiquitin ligase complexes. CRL4 substrates known to be recruited by VprBP include Merlin, Mcm10, UNG2, SMUG1, RORαme1. Known EDD/UBR5 substrates recruited by VprBP include Katanin and TERT. VprBP is involved in a variety of normal cellular processes, including proliferation, DNA replication, cell cycle progression, telomere maintenance, and DNA damage responses. As indicated by its name, VprBP is also a well known target utilized by the Vpr and Vpx accessory factors of primate lentiviruses to highjack the host CRL4VprBP E3 ligase function.

Immunogen

BCP-conjugated linear peptide corresponding an N-terminal sequence of human VprBP.

Epitope: N-terminal region.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected VprBP in 10 µg of HCT116 human colon carcinoma cell lysate.

Specificity

Expected to react with all three spliced isoforms of human VprBP reported by UniProt (Q9Y4B6).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~169 kDa observed. 169.0 kDa (isoform 1), 168.9 kDa (isoform 2), and 118.2 kDa (isoform 3) calculated.

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
clone8A7.2, monoclonal
Gene Informationhuman ... VPRBP(9730)
isotypeIgG2b
NCBI accession no.NP_055518
purified byusing protein G
Quality Level100
shipped inwet ice
species reactivity (predicted by homology)mouse (based on 100% sequence homology)
species reactivityhuman, rat
technique(s)western blot: suitable, immunohistochemistry: suitable (paraffin)
UniProt accession no.Q9Y4B6
This product has met the following criteria to qualify for the following awards:



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